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分子生物学试剂>限制性内切酶>常规限制性内切酶

Reaction Conditions for Restriction Enzymes

Thermo Scientific Enzyme Recommended buffer for
100% activity		 Enzyme activity in 5-buffer system buffers, % Tango buffer for double digestion Units for overnight incubation,
u/μg DNA		 Thermal inactivation in 20 min
B
(blue)
1X		 G
(green)
1X		 O
(orange)
1X		 R
(red)
1X		 Tango
(yellow)
1X / 2X
AanI (PsiI) Tango 50-100 50-100 0-20 0-20 100 20-50 1X or 2X 0.5 65°C
AarI AarI +oligo NR (+oligo) NR (+oligo) 0-20 (+oligo) 0-20 (+oligo) NR (+oligo) 50-100 (+oligo) 2X (+oligo) 0.9 65°C
AatII Tango 50-100 20-50 0-20 0-20 100 20-50 1X or 2X 0.3 65°C
Acc65I (Asp718I) O 0-20 20-50 100 20-50 20-50 50-100 1X or 2X 0.3 65°C
AdeI (DraIII) G 0-20 100 20-50 100 100* 20-50 1X* or 2X 0.5 No
AjiI (BmgBI) AjiI NR NR 20-50* NR NR 20-50* 2X* 0.5 65°C
AjuI R +SAM 0-20 (+SAM) 50-100 (+SAM) 20-50 (+SAM) 100 (+SAM) 50-100 (+SAM) 50-100 (+SAM) 1X or 2X (+SAM) 0.5 65°C
AloI R 0-20 0-20 0-20 100 20-50 100 1X or 2X 0.1 65°C
AluI Tango 50-100 0-20 0-20 0-20 100 20-50 1X or 2X 0.1 65°C
Alw21I (BsiHKAI) O 0-20 20-50 100 50-100 20-50 50-100 1X or 2X 0.1 65°C
Alw26I Tango 50-100 100 0-20 0-20 100 100 1X or 2X 0.2 65°C
Alw44I Tango 50-100 100 0-20 50-100 100 50-100 1X or 2X 0.1 65°C
ApaI B 100 20-50 0-20 0-20 20-50 0-20 1X 0.2 65°C
BamHI BamHI 20-50* 100 20-50 50-100* 100* 50-100 1X* or 2X 0.5 80°C (10u)
BauI (BssSI) Tango 0-20 50-100 0-20 50-100 100 50-100 1X or 2X 0.2 65°C
BclI G 20-50 100 20-50 20-50 100* 100 1X* or 2X 0.1 80°C (10u)
BcnI (NciI) Tango 20-50 50-100 50-100 50-100 100 50-100 1X or 2X 0.2 65°C
BcuI (SpeI) Tango 50-100 50-100 0-20 20-50 100 0-20 1X 0.5 No
BfmI (SfcI) Tango 0-20 50-100 0-20 0-20 100 20-50 1X or 2X 0.9 65°C
BfuI (BciVI) BfuI NR NR 0-20 0-20 NR 50-100* 2X* 0.9 80°C
BglI O 0-20 50-100 100 100 0-20 100 2X 0.1 65°C
BglII O 0-20 20-50 100 50-100 0-20 100 2X 0.1 No
Bme1390I (ScrFI) O 20-50 50-100 100 50-100 50-100 50-100 1X or 2X 0.2 80°C
BoxI (PshAI) Tango 0-20 0-20 0-20 20-50 100 20-50 1X or 2X 0.5 80°C
BpiI (BbsI) G 20-50 100 50-100 50-100 50-100 50-100 1X or 2X 0.3 65°C
BplI Tango 0-20 (+SAM) 20-50 (+SAM) 0-20 (+SAM) 0-20 (+SAM) 100 (+SAM) 20-50 (+SAM) 1X (+SAM) 0.3 65°C
Bpu10I Bpu10I 0-20 20-50* 50-100* 100* 50-100* 100* 1X* or 2X* 0.2 80°C
Bpu1102I (BlpI) Tango 50-100 50-100 20-50 20-50 100 20-50 1X or 2X 0.1 80°C
BseDI (BsaJI) Tango 50-100 20-50 0-20 0-20 100 50-100 1X or 2X 0.2 80°C
BseGI (BtsCI) Tango 20-50 50-100 20-50 20-50 100 20-50 1X or 2X 0.2 80°C
BseJI (BsaBI) O NR 100* 100 NR NR 100* 2X* 0.1 No
BseLI (BslI) Tango 20-50 100 0-20 20-50 100 50-100 1X or 2X 0.1 No
BseMI (BsrDI) R 0-20 20-50 0-20 100 50-100 50-100 1X or 2X 0.3 80°C
BseMII (BspCNI) Tango 50-100 (+SAM) 50-100 (+SAM) 50-100 (+SAM) 50-100 (+SAM) 100 (+SAM) 50-100 (+SAM) 1X or 2X (+SAM) 0.5 80°C
BseNI (BsrI) B 100 20-50 0-20 0-20 50-100 20-50 1X or 2X 0.1 80°C
BseSI (Bme1580I) G 20-50 100 0-20 20-50 50-100 0-20 1X 0.1 80°C (10u)
BseXI (BbvI) BseXI NR NR NR NR NR NR NR 0.3 80°C
Bsh1236I (BstUI) R 0-20 0-20 50-100 100 20-50 50-100 1X or 2X 0.1 65°C
Bsh1285I (BsiEI) G 20-50 100 20-50 0-20 0-20 20-50 2X 0.2 65°C
BshNI (BanI) O 0-20 20-50 100 50-100 0-20 100 2X 0.3 65°C
BshTI (AgeI) O 0-20 20-50 100 50-100 20-50 20-50 1X or 2X 0.1 65°C
Bsp119I (BstBI) Tango 20-50 0-20 0-20 0-20 100 100 1X or 2X 0.1 80°C
Bsp120I (PspOMI) B 100 20-50 0-20 20-50 50-100 0-20 1X 0.1 80°C
Bsp1407I (BsrGI) Tango 0-20 20-50 0-20 20-50 100 50-100 1X or 2X 0.1 65°C
Bsp143I (Sau3AI) Bsp143I 20-50 20-50 0-20 0-20 50-100 20-50 1X or 2X 0.1 65°C
Bsp68I (NruI) O 0-20 20-50 100 50-100 20-50 50-100 1X or 2X 0.1 65°C
BspLI (NlaIV) Tango 50-100 50-100 0-20 20-50 100 20-50 1X or 2X 0.3 65°C
BspOI (BmtI) O 0-20 0-20 100 100 0-20 20-50 2X 0.2 80°C
BspPI (AlwI) Tango 20-50 20-50 0-20 0-20 100 0-20 1X 0.5 80°C
BspTI (AflII) O 0-20 0-20 100 20-50 0-20 50-100 2X 0.1 65°C
Bst1107I (BstZ17I) O 20-50 50-100 100 100 20-50 100 1X or 2X 0.1 65°C
BstXI O 20-50 100 100 50-100 50-100 100 1X or 2X 0.1 65°C
Bsu15I (ClaI) Tango 20-50 20-50 20-50 20-50 100 20-50 1X or 2X 0.1 65°C
BsuRI (HaeIII) R 0-20 0-20 0-20 100 50-100 100 1X or 2X 0.1 80°C
BveI (BspMI) O +oligo 0-20 (+oligo) 20-50 (+oligo) 100 (+oligo) 20-50 (+oligo) 50-100 (+oligo) 100 (+oligo) 1X or 2X (+oligo) 0.2 65°C
CaiI (AlwNI) Tango 20-50 20-50 20-50 50-100 100 50-100 1X or 2X 0.2 65°C
Cfr10I (BsrFI) Cfr10I 0-20 20-50 20-50 50-100 20-50 50-100 1X or 2X 0.1 No
Cfr13I (Sau96I) Tango 50-100 50-100 20-50 20-50 100 20-50 1X or 2X 0.3 65°C
Cfr42I (SacII) B 100 50-100 0-20 0-20 50-100 0-20 1X 0.1 65°C
Cfr9I (XmaI) Cfr9I 0-20 0-20 0-20 0-20 20-50 0-20 1X 0.2 65°C
CpoI (RsrII) Tango 20-50 50-100 50-100 20-50 100 50-100 1X or 2X 0.5 65°C
CseI (HgaI) R NR 50-100* 50-100 100 100* 50-100 1X* or 2X 0.5 80°C (10u)
Csp6I (CviQI) B 100 50-100 0-20 0-20 50-100 0-20 1X 0.1 65°C
DpnI Tango 100 100 50-100 50-100 100 50-100 1X or 2X 0.1 80°C
DraI Tango 50-100 50-100 20-50 20-50 100 50-100 1X or 2X 0.1 65°C
Eam1104I (EarI) Tango 50-100 50-100 0-20 0-20 100 0-20 1X 0.5 65°C
Eam1105I (AhdI) Eam1105I 20-50 50-100 0-20 0-20 50-100 20-50 1X or 2X 0.1 65°C
Ecl136II (EcoICRI) Ecl136II 50-100 20-50 0-20 0-20 50-100 0-20 1X 0.2 65°C
Eco105I (SnaBI) Tango 100* 50-100 0-20 0-20 100 0-20 1X 0.5 65°C
Eco130I (StyI) O 0-20 20-50 100 50-100 50-100 100 1X or 2X 0.2 65°C
Eco147I (StuI) B 100 50-100 20-50 20-50 50-100 0-20 1X 0.1 80°C
Eco24I (BanII) Tango 50-100 50-100 20-50 20-50 100 0-20 1X 0.2 65°C
Eco31I (BsaI) G 50-100 100 0-20 0-20 50-100 20-50 1X or 2X 0.3 65°C
Eco32I (EcoRV) R 0-20 50-100 50-100 100 20-50 100 1X or 2X 0.1 65°C
Eco47I (AvaII) R 0-20 50-100 50-100 100 50-100 50-100 1X or 2X 0.3 65°C
Eco47III (AfeI) O 0-20 20-50 100 100 50-100 100 1X or 2X 0.1 80°C
Eco52I (EagI) Eco52I 0-20 0-20 0-20 20-50 0-20 20-50 2X 0.2 65°C
Eco57I (AcuI) G +SAM 100 (+SAM) 100 (+SAM) 20-50 (+SAM) 20-50 (+SAM) 50-100 (+SAM) 50-100 (+SAM) 1X or 2X (+SAM) 0.9 65°C
Eco72I (PmlI) Tango NR NR 0-20 0-20 100 20-50 1X or 2X 0.5 65°C
Eco81I (Bsu36I) Tango 50-100 100 0-20 0-20 100 0-20 1X 0.1 80°C
Eco88I (AvaI) Tango 100 50-100 0-20 0-20 100 20-50 1X or 2X 0.2 65°C
Eco91I (BstEII) O 20-50 20-50 100 50-100 NR 100 2X 0.1 65°C
EcoO109I (DraII) Tango 50-100 20-50 20-50 20-50 100 100 1X or 2X 0.2 65°C
EcoRI EcoRI 0-20 NR 100 100* NR 100 2X 0.2 65°C
EcoRII O 20-50 50-100 100 50-100 20-50 50-100 1X or 2X 0.1 80°C
EheI (SfoI) Tango 20-50 50-100 0-20 0-20 100 20-50 1X or 2X 0.9 65°C
Esp3I (BsmBI) Tango 100 (+DTT) 20-50 (+DTT) 0-20 (+DTT) 0-20 (+DTT) 100 (+DDT) 0-20 (+DTT) 1X (+DDT) 0.2 65°C
FaqI (BsmFI) Tango 20-50 (+SAM) 20-50 (+SAM) 0-20 (+SAM) 0-20 (+SAM) 100 (+SAM) 50-100 (+SAM) 1X (+SAM) 0.9 80°C
FspAI O 0-20 0-20 100 50-100 0-20 50-100 2X 0.2 65°C
FspBI (BfaI) Tango 50-100 20-50 0-20 0-20 100 0-20 1X 0.3 65°C
GsuI (BpmI) B 100 50-100 0-20 20-50 100 50-100 1X or 2X 0.9 65°C
HhaI Tango 50-100 50-100 20-50 20-50 100 20-50 1X or 2X 0.1 80°C
Hin1I (BsaHI) G 20-50 100 20-50 20-50 20-50 20-50 1X or 2X 0.1 65°C
Hin1II (NlaIII) G 50-100 100 20-50 50-100 50-100 50-100 1X or 2X 0.3 65°C
Hin6I (HinP1I) Tango 50-100 50-100 20-50 20-50 100 50-100 1X or 2X 0.1 65°C
HincII (HindII) Tango 50-100 50-100 20-50 50-100 100 50-100 1X or 2X 0.1 65°C
HindIII R 0-20 20-50 0-20 100 50-100 50-100 1X or 2X 0.1 80°C
HinfI R 0-20 20-50 50-100 100 50-100 50-100 1X or 2X 0.1 65°C
HpaII Tango 50-100 50-100 0-20 20-50 100 20-50 1X or 2X 0.1 65°C
HphI B 100 0-20 0-20 0-20 20-50 0-20 1X 0.1 65°C
Hpy8I (MjaIV) Tango 50-100 50-100 0-20 20-50 100 50-100 1X or 2X 0.1 80°C
HpyF10VI (MwoI) Tango 0-20 0-20 0-20 0-20 100 50-100 1X or 2X 0.1 80°C
HpyF3I (DdeI) Tango 20-50 20-50 20-50 20-50 100 50-100 1X or 2X 0.2 65°C
I-SceI Tango 50-100 50-100 50-100 50-100 100 50-100 1X or 2X 0.9 65°C
Kpn2I (BspEI) Tango 50-100 50-100 0-20 20-50 100 50-100 1X or 2X 0.1 80°C
KpnI KpnI 20-50 0-20 0-20 0-20 20-50 0-20 1X 0.2 80°C
KspAI (HpaI) B 100 50-100* 20-50 20-50 100* 50-100 1X* or 2X* 0.5 65°C
LguI (SapI) Tango 20-50 50-100 20-50 20-50 100 20-50 1X or 2X 0.5 65°C
Lsp1109I (BbvI) Lsp1109I 0-20 20-50* 50-100* 100 20-50* 20-50* 1X* or 2X 0.5 65°C
LweI (SfaNI) Tango 0-20 0-20 0-20 20-50 100 20-50 1X or 2X 0.2 65°C
MauBI Tango 0-20 0-20 0-20 0-20 100 0-20 1X 0.1 65°C
MbiI (BsrBI) Tango 20-50 100 0-20 20-50 100 20-50 1X or 2X 0.2 65°C
MboI R 50-100 50-100 50-100 100 50-100 100 1X or 2X 0.1 65°C
MboII B 100 50-100 20-50 0-20 50-100 20-50 1X or 2X 0.9 65°C
MlsI (MscI) R 0-20 20-50 0-20 100 20-50 50-100 1X or 2X 0.5 65°C
MluI R 0-20 20-50 50-100 100 20-50 50-100 1X or 2X 0.1 65°C
MnlI G 50-100 100 20-50 20-50 20-50 20-50 1X or 2X 0.5 65°C
Mph1103I (NsiI) R 0-20 50-100 20-50 100 50-100 50-100 1X or 2X 0.3 65°C
MreI (Sse232I) G 0-20 100 0-20 0-20 50-100 0-20 1X 0.5 80°C
MspI (HpaII) Tango 50-100 50-100 0-20 0-20 100 50-100 1X or 2X 0.3 65°C
MssI (PmeI) B 100 0-20 0-20 0-20 20-50 0-20 1X 0.5 65°C
MunI (MfeI) G 100 100 0-20 0-20 100 0-20 1X 0.1 65°C
Mva1269I (BsmI) R 0-20 20-50 50-100 100 0-20 50-100 2X 0.1 65°C
MvaI (BstNI) R 20-50 20-50 50-100 100 20-50* 100 1X* or 2X 0.1 80°C (10u)
Nb.Bpu10I R 0-20 20-50 20-50 100 20-50 50-100 1X or 2X 0.3 80°C
Nb.Mva1269I O 0-20 20-50 100 100 20-50 50-100 1X or 2X 0.3 80°C
NcoI Tango 20-50 20-50 20-50 50-100 100 100 1X or 2X 0.1 65°C
NdeI O 0-20 0-20 100 50-100 0-20 50-100 2X 0.3 65°C
NheI Tango 100 20-50 0-20 0-20 100 0-20 1X 0.2 65°C
NmuCI (Tsp45I) R 0-20 20-50 50-100 100 20-50 50-100 1X or 2X 0.1 65°C
NotI O 0-20 20-50 100 20-50 0-20 20-50 2X 0.1 65°C
NsbI (FspI) Tango 20-50 50-100 0-20 20-50 100 20-50 1X or 2X 0.1 65°C
Nt.Bpu10I R 0-20 0-20 100 100 0-20 50-100 2X 0.3 80°C
OliI (AleI) R 0-20 0-20 0-20 100 0-20 50-100 2X 0.2 65°C
PacI PacI 20-50 20-50 0-20 0-20 0-20 0-20 NR 0.3 65°C
PaeI (SphI) B 100 50-100 0-20 0-20 50-100 0-20 1X 0.1 65°C
PagI (BspHI) O 0-20 50-100 100 NR NR NR NR 0.2 80°C
PasI PasI NR NR NR NR NR NR NR 0.2 80°C
PauI (BssHII) R 0-20 0-20 100 100 0-20 100 2X 0.2 80°C
PdiI (NaeI) Tango 50-100 20-50 0-20 0-20 100 50-100 1X or 2X 0.5 65°C
PdmI (XmnI) Tango 20-50 50-100 0-20 0-20 100 0-20 1X 0.5 65°C
PfeI (TfiI) O 0-20 20-50 100 50-100 20-50 50-100 1X or 2X 0.1 65°C
Pfl23II (BsiWI) Tango 20-50 50-100 20-50 20-50 100 0-20 1X 0.3 65°C
PfoI Tango 0-20 20-50 50-100 0-20 100 50-100 1X or 2X 0.1 65°C
Ppu21I (BsaAI) Ppu21I 50-100* 100* 20-50 NR NR NR NR 0.5 65°C
PscI (PciI) Tango 20-50 20-50 0-20 0-20 100 0-20 1X 0.2 65°C
Psp1406I (AclI) Tango 100 50-100 0-20 20-50 100 0-20 1X 0.5 65°C
Psp5II (PpuMI) G 0-20 100 20-50 20-50 50-100 100 1X or 2X 0.2 80°C
PstI O 50-100 50-100 100 100 50-100 50-100 1X or 2X 0.2 80°C (10u)
PsuI (BstYI) B 100 20-50 0-20 0-20 50-100 0-20 1X 0.5 80°C
PsyI (Tth111I) B 100 50-100 0-20 0-20 50-100 0-20 1X 0.1 80°C
PvuI R 0-20 20-50 50-100 100 50-100 100 1X or 2X 0.2 80°C (10u)
PvuII G 50-100* 100 20-50 50-100 20-50* 20-50* 1X* or 2X* 0.2 80°C
RsaI Tango 50-100 20-50 0-20 0-20 100 0-20 1X 0.2 65°C
RseI (MslI) R 0-20 50-100 50-100 100 20-50 100 1X or 2X 0.5 65°C
SacI SacI 50-100 20-50 0-20 0-20 50-100 20-50 1X or 2X 0.2 65°C
SalI O 0-20 0-20 100 20-50 0-20 50-100 2X 0.1 65°C
SatI (Fnu4HI) G 20-50 100 20-50 20-50 50-100 20-50 1X or 2X 0.1 65°C
ScaI ScaI 0-20 0-20 0-20 0-20 0-20 0-20 NR 0.3 80°C (10u)
SchI (MlyI) Tango 20-50 50-100 0-20 0-20 100 0-20 1X 0.2 65°C
SdaI (SbfI) SdaI NR NR 0-20 0-20 NR 20-50 2X 0.3 65°C
SduI (Bsp1286I) SduI NR 50-100* 0-20 0-20 NR NR NR 0.3 65°C
SfaAI (AsiSI) Tango 50-100 0-20 0-20 0-20 100 0-20 1X 0.2 80°C
SfiI G 50-100 100 20-50 0-20 100 0-20 1X 0.2 80°C
SgeI SgeI 0-20 0-20 0-20 NR NR NR NR 0.0 65°C
SgrDI R 0-20 0-20 0-20 100 NR 100 2X 0.3 65°C
SgsI (AscI) Tango 0-20 0-20 0-20 50-100 100 50-100 1X or 2X 0.1 65°C
SmaI Tango 50-100 0-20 0-20 0-20 100 0-20 1X 0.2 65°C
SmiI (SwaI) O 0-20 0-20 100 20-50 0-20 20-50 2X 0.1 65°C
SmoI (SmlI) Tango 50-100 20-50 0-20 20-50 100 20-50 1X or 2X 0.2 80°C
SsiI (AciI) O NR 20-50 100 50-100 NR 100 2X 0.5 65°C
SspDI (KasI) Tango 20-50 0-20 NR 0-20 100 20-50 1X or 2X 1.0 80°C
SspI G 20-50 100 0-20 50-100 100 20-50 1X or 2X 0.1 65°C
TaaI (HpyCH4III) Tango 0-20 0-20 0-20 50-100 100 100 1X or 2X 0.2 No
TaiI (MaeII) R 50-100 50-100 20-50 100 100 50-100 1X or 2X 0.3 No
TaqI TaqI 0-20 20-50 20-50 20-50 20-50 20-50 1X or 2X 0.3 No
TasI (Tsp509I) B 100 50-100 20-50 0-20 20-50 0-20 1X 0.3 No
TatI Tango NR 50-100* 0-20 20-50 100* 0-20 1X* 0.2 No
TauI B 100 50-100 0-20 0-20 20-50 0-20 1X 0.9 No
Tru1I (MseI) R 50-100 50-100 20-50 100 50-100 100 1X or 2X 0.2 No
TscAI (TspRI) Tango 50-100 50-100 20-50 20-50 100 20-50 1X or 2X 0.2 No
Van91I (PflMI) R 0-20 50-100 50-100 100 20-50 50-100 1X or 2X 0.1 65°C
VspI (AseI) O 0-20 50-100 100 20-50 100 100 1X or 2X 0.1 65°C
XagI (EcoNI) R 0-20 20-50 50-100 100 20-50 50-100 1X or 2X 0.1 65°C
XapI (ApoI) Tango 50-100 100 0-20 0-20 100 20-50 1X or 2X 0.1 65°C
XbaI Tango 50-100 50-100 20-50 0-20 100 50-100 1X or 2X 0.1 65°C
XceI (NspI) Tango 50-100 0-20 0-20 0-20 100 0-20 1X 0.2 65°C
XhoI R 0-20 50-100 50-100 100 20-50 100 1X or 2X 0.1 80°C
XmaJI (AvrII) Tango 20-50 50-100 50-100 50-100 100 50-100 1X or 2X 0.2 80°C
XmiI (AccI) B 100 0-20 0-20 0-20 50-100 20-50 1X or 2X 0.1 65°C
产品线推荐:
产品推荐:
品牌 货号 品名
Fermentas ER0011 AluI
Fermentas ER0012 AluI
Fermentas ER0021 Alw21I (BsiHKAI)
Fermentas ER0031 Alw26I(BsmA I,BsoMA I)
Fermentas ER0041 Alw44 I(ApaL I,Vne I)
Fermentas ER0051 BamHI
Fermentas ER0052 BamHI
Fermentas ER0053 BamHI, HC
Fermentas ER0055 BamHI
Fermentas ER0061 BcnI (NciI,AsuC2 I)
Fermentas ER0071 BglI
Fermentas ER0072 BglI
Fermentas ER0081 BglII
Fermentas ER0082 BglII
Fermentas ER0091 Bpu1102I (BlpI)
Fermentas ER0092 Bpu1102I (BlpI)
Fermentas ER0111 Bsp68I (NruI)
Fermentas ER0121 Bsp119I (BstBI)
Fermentas ER0131 Bsp120I (PspOMI)
Fermentas ER0141 Bsu15I (ClaI)
Fermentas ER0142 Bsu15I (ClaI)
Fermentas ER0145 Bsu15I (ClaI)
Fermentas ER0151 BsuRI (HaeIII)
Fermentas ER0171 Cfr9I (XmaI)
Fermentas ER0172 Cfr9I (XmaI)
Fermentas ER0181 Cfr10I (BsrFI)
Fermentas ER0191 Cfr13I (Sau96I)
Fermentas ER0201 Cfr42I (SacII)
Fermentas ER0202 Cfr42I (SacII)
Fermentas ER0205 Cfr42I (SacII)
Fermentas ER0211 Csp6I (CviQI)
Fermentas ER0221 DraI
Fermentas ER0223 DraI, HC
Fermentas ER0231 Eam1104I (EarI)
Fermentas ER0232 Eam1104I (EarI)
Fermentas ER0241 Eam1105I(Ahd I,NruG I,AspE I,EclHK I)
Fermentas ER0251 Ecl136II (EcoICRI)
Fermentas ER0261 EcoO109I (DraII)
Fermentas ER0271 EcoRI
Fermentas ER0272 EcoRI
Fermentas ER0273 EcoRI, HC
Fermentas ER0281 Eco24I (BanII)
Fermentas ER0291 Eco31I (BsaI)
Fermentas ER0292 Eco31I (BsaI)
Fermentas ER0301 Eco32I (EcoRV)
Fermentas ER0302 Eco32I (EcoRV)
Fermentas ER0303 Eco32I (EcoRV), HC
Fermentas ER0305 Eco32I (EcoRV)
Fermentas ER0311 Eco47I (AvaII)
Fermentas ER0312 Eco47I (AvaII)
Fermentas ER0321 Eco47III (AfeI)
Fermentas ER0322 Eco47III (AfeI)
Fermentas ER0331 Eco52I (EagI)
Fermentas ER0332 Eco52I (EagI)
Fermentas ER0341 Eco57I (AcuI)
Fermentas ER0342 Eco57I (AcuI)
Fermentas ER0361 Eco72I (PmlI)
Fermentas ER0371 Eco81I (Bsu36I)
Fermentas ER0372 Eco81I (Bsu36I)
Fermentas ER0381 Eco88I (AvaI)
Fermentas ER0391 Eco91I (BstEII)
Fermentas ER0392 Eco91I (BstEII)
Fermentas ER0401 Eco105I (SnaBI)
Fermentas ER0402 Eco105I (SnaBI)
Fermentas ER0411 Eco130I (StyI)
Fermentas ER0421 Eco147I (StuI)
Fermentas ER0422 Eco147I (StuI)
Fermentas ER0431 ScaI
Fermentas ER0432 ScaI
Fermentas ER0441 EheI (SfoI)
Fermentas ER0451 Esp3I (BsmBI)
Fermentas ER0452 Esp3I (BsmBI)
Fermentas ER0461 GsuI (BpmI)
Fermentas ER0462 GsuI (BpmI)
Fermentas ER0471 Hin1I (BsaHI)
Fermentas ER0481 Hin6I (HinP1I)
Fermentas ER0491 HincII (HindII)
Fermentas ER0492 HincII (HindII)
Fermentas ER0501 HindIII
Fermentas ER0502 HindIII
Fermentas ER0503 HindIII, HC
Fermentas ER0505 HindIII
Fermentas ER0511 HpaII
Fermentas ER0512 HpaII
Fermentas ER0521 KpnI
Fermentas ER0522 KpnI
Fermentas ER0523 KpnI, HC
Fermentas ER0531 Kpn2I (BspEI)
Fermentas ER0532 Kpn2I (BspEI)
Fermentas ER0541 MspI (HpaII)
Fermentas ER0542 MspI (HpaII)
Fermentas ER0551 MvaI (BstNI)
Fermentas ER0561 MluI
Fermentas ER0562 MluI
Fermentas ER0571 NcoI
Fermentas ER0572 NcoI
Fermentas ER0575 NcoI
Fermentas ER0581 NdeI
Fermentas ER0582 NdeI
Fermentas ER0585 NdeI