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分子生物学试剂>分子克隆

在分子水平上提供一种纯化和扩增特定DNA片段的方法。常含有目的基因,用体外重组方法将它们插入克隆载体,形成重组克隆载体,通过转化与转导的方式,引入适合的寄主体内得到复制与扩增,然后再从筛选的寄主细胞内分离提纯所需的克隆载体,可以得到插入DNA的许多拷贝,从而获得目的基因的扩增。
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品牌 货号 品名
Biotechrabbit BR1100300 T4 DNA Ligase Rapid, 600 U/μl
Biotechrabbit BR1100301 T4 DNA Ligase Rapid, 600 U/μl
Biotechrabbit BR1100302 T4 DNA Ligase Rapid, 600 U/μl
Biotechrabbit BR1101101 DNA Blunting Kit
Fermentas B69 10X T4 DNA Ligase Buffer
Fermentas EC0111 Thermo Scientific DH5α Competent Cells for Subcloning
Fermentas EC0112 Thermo Scientific DH5α Competent Cells
Fermentas EC0113 Thermo Scientific DH10B Competent Cells
Fermentas EC0114 Thermo Scientific BL21(DE3) Competent Cells
Fermentas K1214 InsTAclone PCR Cloning Kit
Fermentas K1231 CloneJET PCR Cloning Kit
Fermentas K1232 CloneJET PCR Cloning Kit
Fermentas K1241 aLICator LIC Cloning and Expression Kit 1 (untagged)
Fermentas K1251 aLICator LIC Cloning and Expression Kit 2 (N-terminal His-tag/EK)
Fermentas K1261 aLICator LIC Cloning and Expression Kit 3 (C-terminal His-tag)
Fermentas K1271 aLICator LIC Cloning and Expression Set 1 (All-in-One/EK)
Fermentas K1281 aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WQ)
Fermentas K1291 aLICator LIC Cloning and Expression Set 2 (All-in-One/WQ)
Fermentas K1422 Rapid DNA Ligation Kit
Fermentas K1423 Rapid DNA Ligation Kit
Fermentas K2710 TransformAid Bacterial Transformation Kit
Fermentas K2711 TransformAid Bacterial Transformation Kit
Fermentas R0392 IPTG, dioxane-free
Fermentas R0393 IPTG, dioxane-free
Fermentas R0402 X-Gal
Fermentas R0404 X-Gal
Fermentas R0851 X-Gluc
Fermentas R0852 X-Gluc
Fermentas R0941 X-Gal Solution, ready-to-use
Fermentas R1171 IPTG Solution, ready-to-use
Fermentas SD0041 pBR322 DNA
Fermentas SD0051 pUC18 DNA
Fermentas SD0061 pUC19 DNA
Fermentas SD0141 pTZ19R DNA
Fermentas SD0171 pUC57 DNA
Fermentas SO100 M13/pUC Sequencing Primer (-20), 17-mer
Fermentas SO101 M13/pUC Reverse Sequencing Primer (-26), 17-mer
Fermentas SO113 M13/pUC Sequencing Primer (-40), 17-mer
Fermentas SO114 M13/pUC Sequencing Primer (-46), 22-mer
Fermentas SO115 M13/pUC Reverse Sequencing Primer (-46), 24-mer
Fermentas SO116 SP6 promoter Sequencing Primer, 18-mer
Fermentas SO117 SP6 promoter Sequencing Primer, 24-mer
Fermentas SO118 T7 promoter Sequencing Primer, 20-mer
Fermentas SO120 T3 promoter Sequencing Primer, 24-mer
Fermentas SO501 pJET1.2 Forward Sequencing Primer, 23-mer
Fermentas SO511 pJET1.2 Reverse Sequencing Primer, 24-mer
SMOBIO CK1000 Champion E. coli Transformation Kit, 200 Rxn
SMOBIO CV1000 GetClone PCR Cloning Vector, 20 Rxn
SMOBIO CV1100 GetClone PCR Cloning Vector II, 20 Rxn