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分子生物学试剂>分子克隆

在分子水平上提供一种纯化和扩增特定DNA片段的方法。常含有目的基因,用体外重组方法将它们插入克隆载体,形成重组克隆载体,通过转化与转导的方式,引入适合的寄主体内得到复制与扩增,然后再从筛选的寄主细胞内分离提纯所需的克隆载体,可以得到插入DNA的许多拷贝,从而获得目的基因的扩增。
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品牌 货号 品名
Abnova KA3721 DNA Ligation Kit
Biotechrabbit BR1100300 T4 DNA Ligase Rapid, 600 U/μl
Biotechrabbit BR1100301 T4 DNA Ligase Rapid, 600 U/μl
Biotechrabbit BR1100302 T4 DNA Ligase Rapid, 600 U/μl
Fermentas B69 10X T4 DNA Ligase Buffer
Fermentas K1231 CloneJET PCR Cloning Kit
Fermentas K1232 CloneJET PCR Cloning Kit
Fermentas K1241 aLICator LIC Cloning and Expression Kit 1 (untagged)
Fermentas K1251 aLICator LIC Cloning and Expression Kit 2 (N-terminal His-tag/EK)
Fermentas K1261 aLICator LIC Cloning and Expression Kit 3 (C-terminal His-tag)
Fermentas K1271 aLICator LIC Cloning and Expression Set 1 (All-in-One/EK)
Fermentas K1281 aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WQ)
Fermentas K1291 aLICator LIC Cloning and Expression Set 2 (All-in-One/WQ)
Fermentas K1422 Rapid DNA Ligation Kit
Fermentas K1423 Rapid DNA Ligation Kit
Fermentas R0851 X-Gluc
Fermentas R0852 X-Gluc
Fermentas R0941 X-Gal Solution, ready-to-use
Fermentas R1171 IPTG Solution, ready-to-use
Fermentas SD0041 pBR322 DNA
Fermentas SD0051 pUC18 DNA
Fermentas SD0061 pUC19 DNA
Fermentas SD0141 pTZ19R DNA
Fermentas SD0171 pUC57 DNA
Fermentas SO100 M13/pUC Sequencing Primer (-20), 17-mer
Fermentas SO101 M13/pUC Reverse Sequencing Primer (-26), 17-mer
Fermentas SO113 M13/pUC Sequencing Primer (-40), 17-mer
Fermentas SO114 M13/pUC Sequencing Primer (-46), 22-mer
Fermentas SO115 M13/pUC Reverse Sequencing Primer (-46), 24-mer
Fermentas SO116 SP6 promoter Sequencing Primer, 18-mer
Fermentas SO117 SP6 promoter Sequencing Primer, 24-mer
Fermentas SO118 T7 promoter Sequencing Primer, 20-mer
Fermentas SO119 T3 promoter Sequencing Primer, 17-mer
Fermentas SO120 T3 promoter Sequencing Primer, 24-mer
Fermentas SO501 pJET1.2 Forward Sequencing Primer, 23-mer
Fermentas SO511 pJET1.2 Reverse Sequencing Primer, 24-mer
SMOBIO CK1000 Champion? E. coli Transformation Kit, 200 Rxn
SMOBIO CV1000 GetClone? PCR Cloning Vector, 20 Rxn
SMOBIO CV1100 GetClone? PCR Cloning Vector II, 20 Rxn