β淀粉样蛋白检测试剂盒
阿尔茨海默病(Alzheimer’s Disease, AD)以脑内细胞外老年斑(senile plaques,SPs)和细胞内神经原纤维缠结(neurofibrillary tangles,NET)的出现为病灶。老年斑(SPs)的主要组成蛋白是β淀粉样多肽(Amyloid peptide,Aβ)40 和42。β淀粉样蛋白42(Aβ42)较β淀粉样蛋白40(Aβ40)更易聚积,因此,Aβ沉积开始于Aβ42 而非Aβ40。Aβ42 阳性和Aβ40 阴性斑可代表早期扩散??老年斑,Aβ40 阳性斑出现在中晚期,特别是老年斑的核心部分。
Wako的AβElisa Kit提高了检测敏感性,不仅能应用于组织培养上清液(tissue culture medium)、组织匀浆(tissue homogenate)、脑脊髓液(cerebrospinal fluid,CSF)样品,还可应用于血浆(plasma),并采用了片断化的抗体,提高了抗原抗体结合力,减少假阳性结果的产生。
BAN50:特异检测Aβ N 末端
BNT77:特异检测Aβ(11-28)
BA27:特异检测Aβ40 C 端
BC05:特异检测Aβ42 C 端
β淀粉样蛋白40 42 第一代ELISA 检测试剂盒

货号
产品描述
规格
用途
Human βAmyloid(1-42) ELISA Kit
96T
定量检测人Aβ(1-42
290-62601
Human/Rat βAmyloid(42)ELISA Kit
96T
定量检测人、大鼠、小鼠N 末端剪切或修饰的Aβ(X-42
292-62301
Human βAmyloid(1-40) ELISA Kit
96T
定量检测Aβ(1-40
294-62501
Human/Rat βAmyloid(40)ELISA Kit
96T
定量检测人、大鼠、小鼠N 末端剪切或修饰的Aβ(X-40

WAKO 针对上述试剂盒,新推出了更加敏感和稳定的第二代试剂盒

货号
产品描述
规格
用途
参考价格
296-64401
Human βAmyloid(1-42) ELISA Kit Wako, High Sensitive
96T
定量检测人Aβ(1-42
292-64501
Human/RatβAmyloid(42) ELISA Kit Wako, High Sensitive
96T
定量检测人、大鼠、小鼠N 末端剪切或修饰的
Aβ(X-42
298-64601
Human βAmyloid(1-40) ELISA Kit Wako II
96T
定量检测Aβ(1-40
294-64701
Human/Rat βAmyloid(40) ELISA Kit Wako II
96T
定量检测人、大鼠、小鼠N 末端剪切或修饰的
Aβ(X-40

产品简介
1. 高敏感性β淀粉样蛋白(1-42和(X-42检测试剂盒
此试剂盒用于高敏感性检测Aβ(1-42)和Aβ(X-42)(在β淀粉样多肽的N 末端做修饰或剪切)。β淀粉样蛋白与阿尔茨海默病有关。此试剂盒的检测敏感性是298-62401⑤和290-62601⑥的10 倍,动态范围(dynamic range)为0.1-20.0pmol/L,使用了标记抗体的Fab’片断,非特异性结合减少。
特点:
1、高敏感性检测Aβ(1-42)和Aβ(X-42):动态范围(dynamic range):0.1-20.0pmol/L;
2、使用了单克隆抗体BC05(Fab’片断),可特异性检测Aβ42 的C 末端;
3、高特异性单抗由武田制药有限公司(Takeda Pharmaceutical Company Ltd.)提供。
试剂盒组成:(①、②、③、④)
1.抗体包被微孔板(ELISA 板) 1 块
2.标准溶液 2ml×2 管
3.标准稀释液 30ml×1 管
4.洗涤液 50ml×1 管
5.HRP 标记的抗体溶液 12ml×1 管
6.TMB 溶液 12ml×1 管
7.终止液 12ml×1 管
8.封板条(Plate seal) 3 片
2. 改进的β淀粉样蛋白(1-40和(X-42检测试剂盒
此试剂盒利用了标记抗体BA27(能识别Aβ40 C 末端)的F(ab’)2 片断,提高了抗原-抗体反应稳定性,
降低了非特异性结合。因此,在洗涤液中稳定性提高,人/大鼠试剂盒的反应背景降低。
3. 使用方法:
1、加入100ul 标准溶液和样品入ELISA 板中,用封板条封口后放冰箱中孵育过夜;
2、洗涤;
3、加入100ulHRP 标记的抗体溶液,封板后入冰箱孵育1 小时;
4、洗涤5 次;
5、加入100ulTMB 显色液,封板后,在暗处室温下孵育30 分钟
6、加入100ul 终止液,在450nm 处读吸光度。
4. 标准曲线
5. 敏感度、动态范围

货号
产品名称
灵敏度 (pg/ml)
动态范围 (pg/ml)
292-62301
Human Beta-Amyloid (1-40) ELISA Kit
0.52(=0.12pM)
4.3-430(=1.0-100pM)
298-64601
Human Beta-Amyloid(1-40) ELISA Kit II
0.082 (=0.019pM)
4.3-430(=1.0-100pM)
298-62401
Human Beta-Amyloid (1-42) ELISA Kit
0.36(=0.08pM)
4.5-450(=1.0-100pM)
296-64401
Human Beta-Amyloid (1-42) ELISA Kit, High Sensitive
0.27(=0.06pM)
0.45-90.0(=0.1-20.0pM)
294-62501
Human/ Rat Beta-Amyloid (40) ELISA Kit
1.08(=0.25pM)
4.30-430(=1.0-100pM
294-64701
Human/ Rat Beta-Amyloid (40) ELISA Kit II
0.21(=0.049pM)
0.43-86.0(=0.1-20.0pM)
290-62601
Human/ Rat Beta-Amyloid (42) ELISA Kit
0.857(=0.19pM)
4.50-450(=1.0-100pM)
292-64501
Human/ Rat Beta-Amyloid (42) ELISAKit, High-Sensitive
0.108(=0.024pM)
0.45-90.0(=0.1-20.0pM)

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