Phusion DNA Polymerases




概述


自从2003年问世以来,Thermo Scientific Phusion高保真DNA聚合酶建立了高保真PCR的新标准。Phusion DNA 高保真DNA聚合酶也逐渐成为更多PCR用户的第一选择,尤其是对这些基因组功能研究的客户来说,必不可少。


Phusion 高保真DNA聚合酶,是一种具有校对功能的DNA聚合酶,来源于类热球菌(Pyrococcus-like),融合有DNA结合域。借助这种融合技术,Phusion DNA聚合酶具有一般单酶无法实现的PCR扩增的速度和准确率,即使是扩增复杂模板也是效率很高。除此之外,Phusion DNA聚合酶对多种抑制剂具有很强的耐受性,只需要极少的优化步骤就可以实现PCR的有效扩增,对于热启动PCR有需求的客户来说,Phusion Hot Start II DNA Polymerase是您的理想选择。

Phusion高保真DNA聚合酶宣传册

产品特点


● 精确-是目前保真度最高的热稳定性聚合酶(是Taq的50倍, Pfu的6倍)


● 强劲-更高的成功率和更少的优化步骤


● 快速-延伸速率为15-30s/kb


● 高产-低酶量、高产量


● 特异-独特的热启动修饰,实现“零时间”激活,减少了非特异性扩增和引物降解


产品应用

高保真PCR、快速PCR、热启动PCR、长片段扩增、高通量PCR

PCR扩增的高保真性


在很多应用中,比如,定点突变或者从DNA到蛋白的翻译过程中,维持DNA序列的准确性在PCR扩增过程中是很关键的。一个错误的核酸掺入将会改变编码序列从而改变翻译后的蛋白质氨基酸序列,这样将影响蛋白的折叠及功能特性。另一方面,一个氨基酸的缺失将会破坏整个正确的读码框。

Phusion DNA 聚合酶具有极低的错赔率,从而建立了Taq  PCR反应的金标准,用基于修饰的lacI方法检测结果显示Phusion DNA 聚合酶具有50倍以上Taq DNA聚合酶及大于6倍的Pfu DNA的保真度。(see Figure 1).

bar graph showing relative fidelity values of different DNA polymerases

Figure 1. Relative fidelity values of different DNA polymerases. Fidelity = 1 / error rate. The low error rate of Phusion DNA Polymerase was recently confirmed by studies using 454 sequencing2 and Illumina sequencing3. With 454 sequencing, Phusion DNA Polymerase was shown to have the lowest error rate compared to other DNA polymerases (see Table 1).

Table 1. Error rates determined by 454 sequencing for seven different DNA polymerases after PCR amplification of four different exons from the human TP53 oncogene, using a clonal TP53 plasmid as starting template.



a Error rate, number of errors (miscalled bases, inserted or deleted bases) divided by total number of bases.bDot, three successive negative flows during 454 sequencing. Table reprinted with the permission from the corresponding author and the journal editor.
B.F. Frey & B. Suppmann (1995) Demonstration of the Expand PCR System's greater fidelity and higher yields with a lacI-based fidelity assay. Biochemica 2, 34-35.Vandenbroucke et al. (2011) Minor variant detection in amplicons using 454 massive parallel pyrosequencing: experiences and considerations for successful applications. Biotechniques. 53:167-177.Kinde et al. (2011) Detection and quantification of rare mutations with massively parallel sequencing. PNAS. 108(23):9530–9535.


产品信息:


货号

产品名称

规格

目录价¥

Phusion高保真PCR

F-530S

Phusion High-Fidelity DNA Polymerase

100 U(2U/ul)

1027

F-530L

Phusion High-Fidelity DNA Polymerase

500 U(2U/ul)

4077

F-534S

Phusion Green High-Fidelity DNA Polymerase

100 Unit(2U/ul)

1027

F-534L

Phusion Green High-Fidelity DNA Polymerase

500 Unit(2U/ul)

4077

F-553S

Phusion High-Fidelity PCR Kit

50 x 50 µL rxns

644

F-553L

Phusion High-Fidelity PCR Kit

200 x 50 µL rxns

2178

F-532S

Phusion High-Fidelity PCR Master Mix with GC Buffer

100 x 50 µL rxns

1657

F-532L

Phusion High-Fidelity PCR Master Mix with GC Buffer

500 x 50 µL rxns

6616

F-531S

Phusion High-Fidelity PCR Master Mix with HF Buffer

100 x 50 µL rxns

1657

F-531L

Phusion High-Fidelity PCR Master Mix with HF Buffer

500 x 50 µL rxns

6616

Phusion高保真快速PCR Mix

F-548S

Phusion Flash High-Fidelity PCR Master Mix

100 rxns

772

F-548L

Phusion Flash High-Fidelity PCR Master Mix

500 rxns

3094

Phusion 高保真热启动DNA聚合酶及Mix

F-549S

Phusion Hot Start II High-Fidelity DNA Polymerase

100 U(2U/ul)

1245

F-549L

Phusion Hot Start II High-Fidelity DNA Polymerase

500 U(2U/ul)

4951

F-537S

Phusion Green HSII HF DNA pol

100 Unit(2U/ul)

1245

F-537L

Phusion Green HSII HF DNA pol

500 Unit(2U/ul)

4951

F-565S

Phusion Hot Start II High-Fidelity PCR master Mix

100 x 50 µL rxns

1715

F-565L

Phusion Hot Start II High-Fidelity PCR master Mix

500 x 50 µL rxns

6834

F-566S

Phusion Green Hot Start II High-Fidelity PCR master Mix

100 x 50 µL rxns

1715

F-566L

Phusion Green Hot Start II High-Fidelity PCR master Mix

500 x 50 µL rxns

6834

Phusion高保真定点突变试剂盒

F-541

Phusion Site-Directed Mutagenesis Kit

20 rxns

1455

Phusion U高保真酶及Mix

F-556S

Phusion U Green HS Polymerase

100 Unit(2U/ul)

1397

F-556L

Phusion U Green HS Polymerase

500 Unit(2U/ul)

5523

F-564S

Phusion U Green Multiplex PCR Master Mix

100 x 50 µL rxns

1959

F-564L

Phusion U Green Multiplex PCR Master Mix

500 x 50 µL rxns

7784

F-555S

Phusion U HS DNA Polymerase

100 Unit(2U/ul)

1397

F-555L

Phusion U HS DNA Polymerase

500 Unit(2U/ul)

5523

F-533S

Phusion U HS PCR Master Mix

100 x 50 µL

1985

F-533L

Phusion U HS PCR Master Mix

500 x 50 µL

7893

F-562S

Phusion U Multiplex PCR Master Mix

100 x 50 µL rxns

1959

F-562L

Phusion U Multiplex PCR Master Mix

500 x 50 µL rxns

7784

Phusion高保真DNA聚合酶Buffer

F-519L

Phusion GC Buffer Pack

4 x 1.5 mL

152

F-521L

Phusion GC Buffer Pack, Detergent-Free

4 x 1.5 mL

152

F-518L

Phusion HF Buffer Pack

4 x 1.5 mL

152

F-520L

Phusion HF Buffer Pack, Detergent-Free

4 x 1.5 mL

152

F-539L

Phusion Green GC Buffer Pack

4 x 1.5 mL

152

F-538L

Phusion Green HF Buffer Pack

4 x 1.5 mL

152

 

 

 

 

 

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